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Dextran Sulfate Sodium Salt (DSS) - Colitis Grade (36,000-50,000 MW)

Dextran Sulfate Sodium Salt (DSS) - Colitis Grade (36,000-50,000 MW)

Synonyms DSS, Dextran Sodium Sulfate, Dextrane Sulfate Sodium Salt

Molecular Weight: 165.192 g/mol

MDL Number: MFCD00081551

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SKU 02160110-CF
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Description

Product Description

Dextran sulfate sodium salt, or DSS, is a branched glucan that triggers colitis in mice and rats when administered in drinking water. DSS binds to medium-chain-length fatty acids in the colon and induces intestinal inflammation. Dextran sulfate sodium is also used to precipitate lipoproteins and to release DNA from DNA-histone complexes.


Learn more about MP Biomedicals DSS with these articles from the National Library of Medicine from the National Institute of Health and the Nature Protocols Research Journal.

Application Notes

Dextran Sulfate Sodium (DSS) is proven to have versatile value in biomedical/clinical research. DSS can be used to study colitis (Inflammatory Bowel disease) in research animals and can also be used as an antiviral agent. Inflammatory Bowel Disease (IBD) is characterized by chronic and relapsing inflammation of the gastrointestinal tract and is associated with increased risk of developing colitis-associated cancer. Several animal models have been used to study colitis and one such method involves the oral administration of dextran sulfate sodium (DSS) in the drinking water of mice, leading to chronic colitis. This DSS induced colitis model is spontaneous and is used to assess the therapeutic potential of treatments for IBD.


Bamba et al (2012) performed a comparative analysis of 3 different DSS preparations to examine the chemical and cytotoxic properties, as well as the severity of colitis. Their study concluded that the DSS from MP Bio induced severe colitis as indicated by body weight transition, DAI score, colon weight/length and histological scores. This DSS is well characterized and offers the highest sulfur content (19%), highest chirality (+104 ° ° of specific rotation), and lowest pH (6.2 at 1% solution).


Bamba S, Andoh A, Ban H, et al: The severity of dextran sodium sulfate-induced colitis can differ between dextran sodium sulfate preparations of the same molecular weight range. Dig Dis Sci. 57:327-334. 2012

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals

Key Applications

Colitis, Antilipemic


Critical parameters and key factors in applications utilizing DSS for colitis research are discussed in an article in Current Protocols in Immunology: "The successful and reproducible induction of DSS-induced colitis depends on numerous key factors, including DSS source, lot #, molecular weight, concentration, duration, mouse strain, source, age, gender and body weight as well as environmental factors including the hygienic condition of the vivarium1. If high mortality is observed, suggesting high susceptibility to DSS, a decreased dose of DSS should be adopted. If no or weak colitis is observed, suggesting low susceptibility, and increase in DSS concentration/and or duration should be considered."


Nell S, Suerbaum S, Josenhans C. The impact of the microbiota on the pathogenesis of IBD: lessons from mouse infection models. Nature reviews. Microbiology. 2010;8:564-577.

Specifications
SKU 02160110-CF
Application Notes <p>Dextran Sulfate Sodium (DSS) is proven to have versatile value in biomedical/clinical research. DSS can be used to study colitis (Inflammatory Bowel disease) in research animals and can also be used as an antiviral agent. Inflammatory Bowel Disease (IBD) is characterized by chronic and relapsing inflammation of the gastrointestinal tract and is associated with increased risk of developing colitis-associated cancer. Several animal models have been used to study colitis and one such method involves the oral administration of dextran sulfate sodium (DSS) in the drinking water of mice, leading to chronic colitis. This DSS induced colitis model is spontaneous and is used to assess the therapeutic potential of treatments for IBD.</p><br><p>Bamba et al (2012) performed a comparative analysis of 3 different DSS preparations to examine the chemical and cytotoxic properties, as well as the severity of colitis. Their study concluded that the DSS from MP Bio induced severe colitis as indicated by body weight transition, DAI score, colon weight/length and histological scores. This DSS is well characterized and offers the highest sulfur content (19%), highest chirality (+104 ° ° of specific rotation), and lowest pH (6.2 at 1% solution).</p><br><p>Bamba S, Andoh A, Ban H, et al: The severity of dextran sodium sulfate-induced colitis can differ between dextran sodium sulfate preparations of the same molecular weight range. Dig Dis Sci. 57:327-334. 2012</p>
Boiling Point 310 ° C
Grade Colitis grade
Applications <p>Colitis, Antilipemic</p><br><p>Critical parameters and key factors in applications utilizing DSS for colitis research are discussed in an article in <a href="https://currentprotocols.onlinelibrary.wiley.com/doi/abs/10.1002/0471142735.im1525s104">Current Protocols in Immunology</a>: "The successful and reproducible induction of DSS-induced colitis depends on numerous key factors, including DSS source, lot #, molecular weight, concentration, duration, mouse strain, source, age, gender and body weight as well as environmental factors including the hygienic condition of the vivarium1. If high mortality is observed, suggesting high susceptibility to DSS, a decreased dose of DSS should be adopted. If no or weak colitis is observed, suggesting low susceptibility, and increase in DSS concentration/and or duration should be considered."</p><br><p>Nell S, Suerbaum S, Josenhans C. The impact of the microbiota on the pathogenesis of IBD: lessons from mouse infection models. Nature reviews. Microbiology. 2010;8:564-577.</p>
Melting Point 92 ° C
Molecular Weight 165.192 g/mol
Pack Size No
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Physical Appearance White Powder
Product Families Description Dextran sulfate sodiium salt
Product Overview Dextran Sulfate Sodium Salt (36,000-50,000 M.Wt.) Colitis Grade
References <p>Kinchen, J. Structural Remodeling of the Human Colonic Mesenchyme in Inflammatory Bowel Disease. Cell. 2018, 175, 372-386.<br>Mahalhal , A. Oral iron exacerbates colitis and influences the intestinal microbiome, PLOS ONE. 2018, 13, e0202460.<br>Dokoshi, T. Hyaluronidase inhibits reactive adipogenesis and inflammation of colon and skin. JCI Insight. 2018, 3, e12307.<br>Zhu, H. RNA virus receptor Rig-I monitors gut microbiota and inhibits colitis-associated colorectal cancer. J. Exp. Clin. Cancer Res. 2017, 36, 2.<br>Monticelli, L. IL-33 promotes an innate immune pathway of intestinal tissue protection dependent on amphiregulin–EGFR interactions. PNAS. 2015, 112, 10762-10767.</p>
Residue on Ignition <50%
RTECS Number HH9290000
Solubility >24.8 [ug/mL]
Storage and Handling Store at Room Temperature (15-30 ° C)
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals
Vapor Pressure 2.6 x 10-4 mm Hg at 25 ° ° C (Estimated)